Effects of metals on elastase fromPseudomonas aeruginosa SES-938-1 |
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Authors: | Semra Kocabiyik Ezgi Ergin Suna Turkoglu |
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Affiliation: | (1) Department of Biology, Middle East Technical University, 06531 Ankara, Turkey |
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Abstract: | Among the numerous virulance factors produced byPseudomonas aeruginosa, elastase is the one most often associated with pathogenesis. In this study, effects of various metal ions on elastase from a new isolate ofP. aeruginosa (Strain SES-938-1) was investigated. Crude elastase was prepared from culture supernatant via salting out by ammonium sulfate, and then desalting and concentrating the sample using a centricon microconcentrator. Activities were measured at 450 nm usingN-succinyl-l-(ala)3-p-nitroanilide as the substrate. The metal chelating agents EDTA and EGTA inhibited thePseudomonas elastase, which shows that the enzyme is a typical metalloproteinase. At a 10-mM concentration, Mn2+, Ni2+, and Zn2+ strongly inhibited the elastase, whereas Mg2+ effect was negligable. There was a gradual decrease in the enzyme activity in accordance with an increase in the concentration of metal ions. |
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Keywords: | Elastase metallo-proteinase Pseudonomas aeruginosa |
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