Amino-acid biosynthesis in the cotyledons of Sinapis alba L. in darkness and far-red light studied by deuterium labelling and mass spectrometry |
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| Authors: | N. M. Shaw K. R. Parsley D. D. Davies |
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| Affiliation: | (1) School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK;(2) Mass Spectrometry Unit, AFRC Food Research Institute, Colney Lane, NR4 7UA Norwich, UK |
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| Abstract: | The incorporation of deuterium from deuterium oxide into the free amino acids of the cotyledons of Sinapis alba L. was studied by gas chromatography-mass spectrometry and was similar, both qualitatively and quantitatively, after incubation of the seedlings in darkness or far-red light. The results support studies which show that phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is synthesised de novo, rather than activated, in response to far-red light.Abbreviations GC-MS Gas chromatography-mass spectrometry - PAL phenylalanine ammonia-lyase (EC 4.3.1.5) - HFB n-propyl heptafluorobutyryl n-propyl |
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| Keywords: | Amino acid and amino-acid synthesis Phytochrome (synthesis) Sinapis (amino acid, phytochrome) |
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