Hepatocyte Growth Factor (HGF)-Induced Cell Migration Is Negatively Modulated by Epidermal Growth Factor through Tyrosine Phosphorylation of the HGF Receptor |
| |
Authors: | Kenji Takeuchi Sayumi Shibamoto Makio Hayakawa Takamitsu Hori Keiji Miyazawa Naomi Kitamura Fumiaki Ito |
| |
Affiliation: | aDepartment of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, 573-01, Japan;bInstitute for Liver Research, Kansai Medical University, Moriguchi, Osaka, 570, Japan |
| |
Abstract: | Hepatocyte growth factor (HGF) stimulated cell migration of human gastric carcinoma cell lines MKN1, MKN7, and MKN28. Epidermal growth factor (EGF) also stimulated the cell migration of these three cell lines. In MKN7 cells, HGF-stimulated cell migration was rather reduced in the presence of EGF, whereas such an observation was not made with MKN1 and MKN28 cells. Therefore, we compared the effect of EGF on HGF-stimulated HGF receptor phosphorylation in these cell lines. HGF induced a rapid tyrosine phosphorylation of the HGF receptor in all these cell lines. In MKN7 cells, the increased phosphorylation was further enhanced by EGF, although EGF alone did not affect tyrosine phosphorylation of the HGF receptor. In MKN1 and MKN28 cells, EGF did not influence tyrosine phosphorylation of the HGF receptor, whether HGF was present or not. The data presented here suggest that EGF negatively modulates the cellular response to HGF by increasing tyrosine phosphorylation of the HGF receptor in certain types of epithelial cells, e.g., MKN7 cells. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|