Production and characterization of the monoclonal antibodies to <Emphasis Type="Italic">Bacillus anthracis</Emphasis> protective antigen |
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| Authors: | N V Rudenko S G Abbasova E V Grishin |
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| Institution: | 1.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch,Russian Academy of Sciences,Moscow oblast,Russia;2.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences,Moscow,Russia |
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| Abstract: | Anthrax, a cosmopolitan acute infectious disease affecting animals and humans, is a category A bioterrorism threat agent because
of the high resistance of Bacillus anthracis spores to adverse environmental factors and their easy production. In this work, we have obtained a representative panel
of 20 monoclonal antibodies to the anthrax protective antigen, a key component of the pathogenic exotoxin. A sandwich ELISA
for quantification of the protective antigen using these antibodies was developed. Six pairs of monoclonal antibodies are
able to detect the protective antigen in blood serum with a detection limit of 1 ng/ml. |
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