A set of near-isogenic lines of Indica-type rice variety CO 39 as differential varieties for blast resistance |
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Authors: | Mary?Jeanie?Telebanco-Yanoria Yohei?Koide Yoshimichi?Fukuta Tokio?Imbe Hiroshi?Tsunematsu Hiroshi?Kato Leodegario?A?Ebron Thi?Minh?Nguyet?Nguyen Email author" target="_blank">Nobuya?KobayashiEmail author |
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Institution: | (1) International Rice Research Institute, DAPO Box 7777 Metro Manila, Philippines;(2) Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba Ibaraki, 305-8686, Japan;(3) National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi Kumamoto, 861-1192, Japan;(4) National Institute of Crop Science, National Agriculture and Food Research Organization, 3-1-1 Kannondai, Tsukuba Ibaraki, 305-8517, Japan;(5) Agricultural Genetics Institute, Conhue, Tuliem, Hanoi, Vietnam; |
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Abstract: | Twenty-seven near-isogenic lines (NILs) with the genetic background of a blast-susceptible variety, CO 39, were developed
by repeated backcrossing as a first set of a large number of differential varieties (DVs) with Indica-type genetic background.
The NILs included 14 resistance genes—Pish, Pib, Piz-5, Piz-t, Pi5(t), Pik-s, Pik, Pik-h, Pik-m, Pik-p, Pi1, Pi7(t), Pita, and Pita-2—derived from 26 donor varieties. The reaction patterns of NILs against 20 standard isolates from the Philippines were similar
to those of blast monogenic lines with the same resistance gene, except for those against two isolates that are avirulent
to Pia in the genetic background of CO 39. A genome-wide DNA marker survey revealed that chromosome segments were introgressed in
the regions where each resistance gene was previously mapped and most of the other chromosome regions in each NIL were CO
39 type. Segregation analysis of resistance and co-segregation analysis between resistance and DNA markers using F3 populations
derived from the crosses between each NIL and the recurrent parent, CO 39, revealed a single-gene control of resistance and
association between resistance and target introgressed segments. The morphological characters of each NIL were almost the
same as those of the recurrent parent except for some lines, suggesting that these NILs can be used even under tropical conditions
where Japonica-type DVs are not suitable for cropping. Thus, these NILs are useful not only as genetic tools for blast resistance
study but also as sources of genes for breeding of Indica-type rice varieties. |
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