Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium |
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| Authors: | Z Pietrzkowski K Reiss W Korohoda |
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| Affiliation: | Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland. |
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| Abstract: | The supplementation of serum-free Eagle's minimal essential medium (EMEM) with Dextran T-500 significantly improves attachment, spreading and survival of chick embryo cells in primary (myoblasts) and secondary (fibroblasts) cultures. These effects were observed in quiescent cultures incubated at 4 degrees C as well as at 37 degrees C. The results suggest that dextran can be used to simplify conditions for research on factors influencing cell proliferation and differentiation in serum-free media. |
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