Type II isopentenyl diphosphate isomerase from Synechocystis sp. strain PCC 6803 |
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Authors: | Barkley Sam J Desai Shrivallabh B Poulter C Dale |
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Affiliation: | Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA. |
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Abstract: | Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni(2+) chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg(2+) for activity; K(m)(IPP) was 52 microM, and k(cat)(IPP) was 0.23 s(-1). |
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