Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis |
| |
| Authors: | Noriaki Takemoto Takuji Yoshimura Satsuki Miyazaki Fumi Tashiro Jun-ichi Miyazaki |
| |
| Affiliation: | 1. Division of Stem Cell Regulation Research, Osaka University Graduate School of Medicine, 2–2 Yamadaoka, Suita, Osaka, 565–0871, Japan;2. Laboratory of Reproductive Engineering, Institute of Experimental Animal Sciences, Osaka University Medical School, 2–2 Yamadaoka, Suita, Osaka, 565–0871, Japan;Zhejiang University, CHINA |
| |
| Abstract: | The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and {"type":"entrez-nucleotide","attrs":{"text":"BC048502","term_id":"28913420"}}BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice. |
| |
| Keywords: | |
|
|
