Ficin E,a serine-centred protease from Ficus elastica |
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| Institution: | 1. School of Pharmacy, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia;2. School of Pharmacy, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK;3. School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia;4. Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia;5. Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia;1. Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al Madinah Al Munawwarah, 30078, Saudi Arabia;2. Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt;3. Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia;4. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt;5. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia;6. Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt;7. Pharmacognosy Department, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, 71524, Egypt;1. Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), 35900, Tanjong Malim, Perak, Malaysia;2. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310, Skudai, Johor, Malaysia;3. Faculty of Applied Sciences, Universiti Teknologi MARA, Pasir Gudang Campus, Jalan Purnama, Bandar Seri Alam, 81750 Masai, Johor, Malaysia;4. Kulliyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia;5. Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, United Kingdom;6. Laboratory of Growth Regulators, Institute of Experimental Botany ASCR & Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech Republic |
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| Abstract: | A protease of M, 50 000 was purified from the latex of Ficus elastica. The enzyme has a pI of 3.7 and pH optimum at 6.0. Its activity is dependent on serine and histidine residues. The amino acid composition is reported. |
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