Carrot phytoalexin inhibits Ca2+, calmodulin-dependent protein phosphorylation in carrot cells |
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| Affiliation: | 1. División de Ginecología y Obstetricia, Hospital Gustavo Baz Prada, Instituto de Salud del Estado de México, Nezahualcóyotl, Estado de México, México;2. Unidad de Investigación en Gerontología, Facultad de Estudios Superiores Zaragoza, UNAM, Ciudad de México, México;1. Department of Animal Biology and Physiology, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon;2. Department of Psychology, University of Yaoundé I, P.O. Box 755, Yaoundé, Cameroon;1. Department of Pediatrics, Furano Kyokai Hospital, Furano, Japan;2. Department of Internal Medicine, Furano Kyokai Hospital, Furano, Japan;3. Department of Clinical Laboratory, Furano Kyokai Hospital, Furano, Japan;1. Escuela de Enfermería, Facultad Medicina y Ciencias de la Salud, Universidad de Barcelona, Barcelona, España;2. Hospital Universitario de Bellvitge, Hospitalet de Llobregat, Barcelona, España;3. Hospital Universitario de Girona Dr. Josep Trueta, Girona, España;4. Hospital Universitario de Vic, Barcelona, España;1. Universidad Nacional de Chilecito (UNdeC), CONICET, Departamento de Ciencias Básicas y Tecnológicas, 9 de Julio 22, Chilecito, F5360CKB, La Rioja, Argentina;2. Instituto de Alta Montaña y Regiones Áridas (IAMRA)- UNdeC, Argentina;3. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco AC, Southeast Unit, CP: 97302, Mérida, Yucatán, Mexico;4. Centro de Investigación Científica de Yucatán, Unidad Recursos Naturales, Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, CP 97205, Mérida, Yucatán, Mexico;5. Centro de Investigación Científica de Yucatán, Unidad Materiales Poliméricos. Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, CP 97205, Mérida, Yucatán, Mexico |
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| Abstract: | Phosphorylation of soluble proteins obtained from cultured carrot cells was monitored by measuring the incorporation of 32P from [32P]ATP into the trichloroacetic acid insoluble fraction. The reaction was stimulated by Ca2+ and calmodulin, and inhibited by a carrot phytoalexin, 6-methoxymellein. 6-Methoxymellein also inhibited the Ca2+, calmodulin-dependent phosphorylative activation of NAD:quinate oxidoreductase (EC 1.1.1.24) partially purified from the carrot cells. The inhibitory effect of 6-methoxymellein was reduced when the reaction mixture contained a high concentration of calmodulin. |
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