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Differential redox proteomics allows identification of proteins reversibly oxidized at cysteine residues in endothelial cells in response to acute hypoxia |
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| Authors: | Izquierdo-Álvarez Alicia Ramos Elena Villanueva Joan Hernansanz-Agustín Pablo Fernández-Rodríguez Rubén Tello Daniel Carrascal Montserrat Martínez-Ruiz Antonio |
| Institution: | Servicio de Inmunología, Hospital Universitario de La Princesa, Instituto de Investigación Sanitaria Princesa (IP), C/Diego de León 62, E-28006 Madrid, Spain. |
| Abstract: | |
| Keywords: | CAM carboxyamidomethylation FDR false discovery rate Grp78 78 kDa glucose-regulated protein HIF hypoxia-inducible factor Hsc70 Heat shock cognate 71 kDa protein Hsp heat shock protein HUVEC human umbilical vein endothelial cells LDH lactate dehydrogenase NEM N-ethylmaleimide OPTM oxidative post-translational modifications PHD HIF prolyl hydroxylase RFS redox fluorescence switch RT room temperature |
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