| 人端粒酶RNA基因的克隆与鉴定 |
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| 引用本文： | 马永红,马永红,张玉静,叶志远,阮承迈,陈守义,吴东林,李鹏. 人端粒酶RNA基因的克隆与鉴定[J]. 生物技术, 2000, 10(6): 5-8 |
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| 作者姓名： | 马永红 马永红 张玉静 叶志远 阮承迈 陈守义 吴东林 李鹏 |
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| 作者单位： | 1. 新疆军区卫生防疫大队,新疆乌鲁木齐830002
2. 解放军军需大学基础部,吉林长春 130062 |
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| 摘 要： | 以人血基因组ＤＮＡ为模板，合成两段２０个寡聚核苷酸为引物，经过ＰＣＲ扩增，得到４８０ｂｐ的片段，克隆到ｐＭＤ１８－Ｔ载体中，经电泳、酶切、ＰＣＲ鉴定后测定序列。序列分析表明氙克隆的人端粒酶ＲＮＡ（ｈｕｍａｎ ｔｅｌｏｍｅａｓｅ ＲＮＡ，ｈＴＲ）基因含有４８０ｂｐ，包括约４５０ｂｐ的编码模板区主序列和约３０ｂｐ的上游调控区序列，其中模板区的１１个核苷酸（５’－ＣＵＡＡＣＣＣＵＡＡＣ－３’）合成端粒亚
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| 关 键 词： | 端粒酶 端粒酶RNA基因 基因克隆 序列鉴定 |
| 文章编号： | 1004-311(2000)06-0005-04 |
| 修稿时间： | 2000-08-24 |
Cloning and characterization of hTR gene |
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| MA Yong-hong ,,ZHANG Yu-jing ,YE Zhi-yuan ,RUAN Cheng-mai ,CHEN Shou-yi ,WU Dong-lin ,LI Peng. Cloning and characterization of hTR gene[J]. Biotechnology, 2000, 10(6): 5-8 |
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| Authors: | MA Yong-hong ZHANG Yu-jing YE Zhi-yuan RUAN Cheng-mai CHEN Shou-yi WU Dong-lin LI Peng |
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| Affiliation: | MA Yong-hong 1,2,ZHANG Yu-jing 2,YE Zhi-yuan 1,RUAN Cheng-mai 2,CHEN Shou-yi 2,WU Dong-lin 2,LI Peng 1 |
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| Abstract: | The chromosome DNA of human blood as template,synthesis 20 oligonucleotides as primer,a fragment which contain 480 base pairs was amplified by PCR and cloned into vector pMD18-T and sequenced.The result of sequence analysis shows this hTR(human telomease RNA) gene fragment containing about 450bp complete template and about 30bp adjacent upstream region regulatory sequence was cloned.The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC-3') complementary to the human telomere sequence (TTAGGG) n.Compared with the known sequence of Feng's hTRcDNA from 293 cells,homology of nucleotide of hTR gene is 100%.So hTR gene has been got. |
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| Keywords: | telomerase hTR gene cloning sequence characterization |
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