氨基酸修饰大孔吸附树脂NBK—110对肿瘤坏死因子吸附性能的研究

应用医用吸附剂直接吸附血浆中的TNFα是一种高效的分离方法,选用8种不同结构和性质的氨基酸修饰大孔吸附树脂NK－110,通过对TNFα吸附量的测定,吸附动力学曲线和吸附等温线的描述等方法,研究了经修饰后大孔吸附树脂对血浆中TNFα的吸附性能,结果表明：1．半胱氨酸饰的NK－110对TNFα的吸附量高,静态吸附120 min时达7683．80u/mL.其吸附动力学曲线表明,经半胱氨酸修饰后,相同时间内吸附量更高,且二者吸附量有差别（P＜0．01）。3．由吸附等温线可见NK－110对血浆中TNFα的吸附量随着溶液浓度的升高而升高,但其吸附率随之呈降低趋势,经半胱氨基酸修饰后,其吸附量随着TNFα浓度的升高而升高,吸附率基本不变。

Adsorption of TNF alpha onto the amino acid-modified NK-110 resin]

It is a effective way to remove Tumor Necrosis Factor(TNF alpha) from plasma by adsorbent. In the present study, NK-110 was modified by 8 amino acids to prepare the adsorbents to be used in the static adsorption experiments of TNF alpha. We have studied the adsorption capacity, kinetic profiles and adsorption isoterm of Cys modified NK-110, and some comparison were made between Cys modified NK-110 and unmodified one. The experimental results show that the Cys modified NK-110 exhibited superior adsorption capacity which is 7683.80 u/mL, and the adsorption percentage is 85.38% at 120 min in stable adsorption. Compared with unmodified NK-110, the Cys-modified one with high adsorption velocity. Furthermore, adsorption isotherms were also studied on Cys-modified and bare NK-110, bot showed to be of "L" shape at 37 degrees C. The adsorption amount increased as the concentration of TNF alpha increased, however, the adsorption percentage is stable adsorbed by Cys-modified NK-110, whereas it is decreased by bare one. The results demonstrating that Cys can significantly raised the adsorption capacity.