Enhancement of rat ACT-1 tumor clonogenicity by xenogeneic mouse macrophages |
| |
Authors: | K W Beagley L S Horne R F X Noronha C M Goodall C M Moore |
| |
Institution: | (1) Cancer Research Unit, University of Otaga Medical School, P.O. Box 913, Dunedin, New Zealand |
| |
Abstract: | Summary In vitro growth of rat atriocaval epithelial tumor cells (ACT-1) was enhanced by the inclusion of xenogeneic mouse adherent
peritoneal exudate cells (PECs) in a two-layer soft agar system. A linear relationship was found between the number of cells
plated and the number of colonies when ACT-1 tumor cells were plated at plating densities of between 1 and 5×105 cell/60 mm plate (r=0.9,P<0.001). Inclusion of irradiated PECs in the bioassay for tumor stem cells resulted in a two and a half-fold increase in colony
formation in three separate experiments (P<0.001).
This work was supported by grants from the Cancer Research Trust, the University of Otago Cancer Research Fund and by the
Medical Research Committee (Golden Kiwi). |
| |
Keywords: | ACT-1 tumor stem cells clonogenicity macrophage growth factors |
本文献已被 SpringerLink 等数据库收录! |