Expression of human pancreatic secretory trypsin inhibitor in Saccharomyces cerevisiae |
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| Authors: | Y Izumoto T Sato T Yamamoto N Yoshida N Kikuchi M Ogawa K Matsubara |
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| Institution: | Institute for Molecular and Cellular Biology, Osaka University, Japan. |
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| Abstract: | Human pancreatic secretory trypsin inhibitor (PSTI) cDNA was expressed in Saccharomyces cerevisiae using the yeast acid phosphatase PHO5 promoter. The product encoded by the PSTI-coding cDNA was correctly processed in yeast cells, and the PSTI molecules were efficiently secreted into the medium. The amino acid composition and the N-terminal amino acid sequence of the secreted PSTI molecules were identical to those of the authentic PSTI polypeptides from human pancreas, and the product exhibited trypsin-inhibitory activity. |
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