Mixture design as a first step for optimization of fermentation medium for cutinase production from <Emphasis Type="Italic">Colletotrichum lindemuthianum</Emphasis> |
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Authors: | Fred J Rispoli Vishal Shah |
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Institution: | (1) Department of Mathematics, Dowling College, Idle Hour Blvd, Oakdale, NY 11955, USA;(2) Department of Biology, Dowling College, Idle Hour Blvd, Oakdale, NY 11955, USA |
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Abstract: | Cutinase enzymes from fungi have found diverse applications in industry. However, most of the available literature on cutinase
production is related to the cultivation of genetically engineered bacteria or yeast cells. In the present study, we use mixture
design experiments to evaluate the influence of six nutrient elements on production of cutinase from the fungus Colletotrichum lindemuthianum. The nutritional elements were starch, glucose, ammonium sulfate, yeast extract, magnesium sulfate, and potassium phosphate.
In the experimental design, we imposed the constraints that exactly one factor must be omitted in each set of experiments
and no factor can account for more than one third of the mixture. Thirty different sets of experiments were designed. Results
obtained showed that while starch is found to have negative influence on the production of the enzyme, yeast extract and potassium
phosphate have a strong positive influence. Magnesium sulfate, ammonium sulfate, and glucose have low positive influence on
the enzyme production. Contour plots have also been created to obtain information concerning the interaction effects of the
media components on enzyme production. |
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Keywords: | Cutinase Colletotrichum lindemuthianum Media optimization Simplex lattice Mixture design |
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