Synthesis of glucose oxidase and catalase by Aspergillus niger in resting cell culture system |
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| Authors: | Liu J Z Yang H Y Weng L P Ji L N |
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| Institution: | Biotechnology Research Center, Zhongshan University, Guangzhou, China. |
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| Abstract: | The synthesis of glucose oxidase and catalase by Aspergillus niger was investigated using a resting cell culture system without growth being established. Calcium carbonate induced the synthesis of both enzymes and calcium chloride inhibited it. The optimal pH for the biosynthesis of glucose oxidase and catalase was 6.0 and 5.7, respectively. The effects of other bivalent cations, reductive compounds and metabolic products on enzyme synthesis were also tested. The biosynthesis of glucose oxidase and catalase was promoted by MnCO3, thioglycolic acid, pyroracemic acid and gluconic acid. |
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