Callus initiation and plant regeneration in ragi (Eleusine coracana Gaertn.) |
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| Authors: | B. D. Mohanty S. Dutta Gupta P. D. Ghosh |
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| Affiliation: | (1) Department of Botany, University of Kalyani, Kalyani, 741235 Nadia, West Bengal, India |
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| Abstract: | Callus was successfully initiated on root, mesocotyl and leaf base segments of 3- to 4-day-old seedlings of ragi (Eleusine coracana Gaertn.). 2,4-D along with casein hydrolysate for Murashige and Skoog's basal medium was found to be most effective for callus initiation and maintenance. Mesocotyl and leaf base tissue derived calli gave shoot buds in medium in which the 2,4-D concentration was lowered. |
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| Keywords: | Eleusine coracana tissue culture plantlet regeneration |
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