New antiherpetic nucleoside from a Basidiomycete |
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Institution: | 1. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, 60971-270 Fortaleza, CE, Brazil;2. Embrapa Agroindústria Tropical, 60511-110 Fortaleza, CE, Brazil;3. Departamento de Química, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil;4. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, 60714-903 Fortaleza, CE, Brazil |
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Abstract: | Antiviral activity was characterized from the culture broth of the Basidiomycete Macrocystidia cucumis (Pers. ex Fr.) Heim. When the stationary phase was reached (21 d), the culture broth was shown through an ELISA assay to contain antiviral activity against herpes simplex virus type 1 (HSV-1), as assessed in baby hamster kidney cells (BHK-21). Once the presence of the anti-HSV-1 activity in the culture broth was demonstrated, we proceeded with the purification and isolation of the active principle using a semi-preparative HPLC technique. The activity was associated with a purine nucleoside designated McA. This compound displayed no cytotoxicity at antivirally effective concentrations and proved to be a novel nucleoside analogue. |
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