Exosomes derived from LPS-stimulated human thymic mesenchymal stromal cells enhance inflammation via thrombospondin-1 |
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Authors: | Qianru Li Jing Li Lei Sun Yun Sun Fei Zhao Pingping Liu Xin Peng Xiaoyan Xuan Yun Li Peng Wang Chen Tan Ying Du |
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Affiliation: | 1.Department of Immunology, School of Basic Medical, Zhengzhou University, Zhengzhou, China;2.Clinical Laboratory, The First Affiliated Hospital of Henan University of CM, Zhengzhou, China |
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Abstract: | Inflammatory response mediated by immune cells is either directly or indirectly regulated by mesenchymal stromal cells (MSCs). Accumulating evidence suggests that thrombospondin-1 (TSP-1) is highly expressed in response to inflammation. In this work, we isolated and identified human thymic mesenchymal stromal cells (tMSCs) and detected the expression of TSP-1. We found that tMSCs expressed TSP-1 and Poly (I:C) or LPS treatment promoted the expression of TSP-1. Further, we isolated and identified exosomes originating from tMSCs (MEXs). Notably, exosomes derived from LPS-pretreated tMSCs (MEXsLPS) promoted the polarization of macrophages to M1-like phenotype and IL-6, TNF-α secretion as well as the pro-inflammatory differentiation of CD4+T cells into Th17 cells. Upon silencing the expression of TSP-1 in tMSCs, the pro-inflammatory effects of MEXsLPS were suppressed. Therefore, these findings uncovered TSP-1 as the principal factor in MEXsLPS pro-inflammatory regulation. |
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Keywords: | exosome inflammation thrombospondin-1 Thymic mesenchymal stem cells |
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