Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine |
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| Authors: | Ruoshi Luo Zhao Qin Dan Zhou Dan Wang Ge Hu Zhiguo Su Suojiang Zhang |
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| Institution: | 1. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing P. R. China ; 2. Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing P. R. China ; 3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing P. R. China |
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| Abstract: | Nylon is a polyamide material with excellent performance used widely in the aviation and automobile industries, and other fields. Nylon monomers such as hexamethylene diamine and other monomers are in huge demand. Therefore, in order to expand the methods of nylon production, we tried to develop alternative bio‐manufacturing processes which would make a positive contribution to the nylon industry. In this study, the engineered E. coli‐overexpressing Lysine decarboxylases (LDCs) were used for the bioconversion of l‐lysine to cadaverine. An integrated fermentation and microfiltration (MF) process for high‐level cadaverine production by E. coli was established. Concentration was increased from 87 to 263.6 g/L cadaverine after six batch coupling with a productivity of 3.65 g/L‐h. The cadaverine concentration was also increased significantly from 0.43 g cadaverine/g l‐lysine to 0.88 g cadaverine/g l‐lysine by repeated batch fermentation. These experimental results indicate that coupling the fermentation and membrane separation process could benefit the continuous production of cadaverine at high levels. |
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| Keywords: | batch fermentation cadaverine lysine decarboxylases membrane separation polyamides monomer |
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