Effects of dexamethasone on human synovial fibroblast-like cells, from osteoarthritic joints, in culture. |
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| Authors: | R Vento M V Torregrossa M Guiliano G Grecomoro F Piccione |
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| Institution: | Institute of Biological Chemistry, University of Palermo, Italy. |
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| Abstract: | The effect of Dexamethasone (DEX) on cell division and macromolecular synthesis was investigated in a line (McCoy cells, A 9) of synovial fibroblast-like cells derived from human osteoarthritic joints. DEX markedly reduced the proliferation of McCoy cells in a time and dose-dependent manner. The maximal inhibition (45%) was found at 500 nM DEX 24 h after incubation and was accompanied by the appearance of giant macrophage-like cells. After DEX treatment cells showed increased content of DNA, proteins and RNA together with the reduction of 3H]-thymidine incorporation into the TCA-precipitable fraction. |
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