Inhibition of microsomal NAD(P)H oxidation by Triton X-100 |
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| Authors: | J A Boutin |
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| Affiliation: | 1. Department of Biology, Faculty of Science, Thaksin University, Phatthalung, Thailand;2. Research and Development Office, Prince of Songkla University, Songkhla, Thailand;3. Chemistry Division, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand;4. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand;5. Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen, Thailand |
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| Abstract: | The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics. |
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