Solution structure and dynamics of human metallothionein-3 (MT-3) |
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Authors: | Wang Hui Zhang Qi Cai Bin Li Hongyan Sze Kong-Hung Huang Zhong-Xian Wu Hou-Ming Sun Hongzhe |
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Institution: | Department of Chemistry and Open Laboratory of Chemical Biology, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China. |
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Abstract: | Alzheimer's disease is characterized by progressive loss of neurons accompanied by the formation of intraneural neurofibrillary tangles and extracellular amyloid plaques. Human neuronal growth inhibitory factor, classified as metallothionein-3 (MT-3), was found to be related to the neurotrophic activity promoting cortical neuron survival and dendrite outgrowth in the cell culture studies. We have determined the solution structure of the alpha-domain of human MT-3 (residues 32-68) by multinuclear and multidimensional NMR spectroscopy in combination with the molecular dynamic simulated annealing approach. The human MT-3 shows two metal-thiolate clusters, one in the N-terminus (beta-domain) and one in the C-terminus (alpha-domain). The overall fold of the alpha-domain is similar to that of mouse MT-3. However, human MT-3 has a longer loop in the acidic hexapeptide insertion than that of mouse MT-3. Surprisingly, the backbone dynamics of the protein revealed that the beta-domain exhibits similar internal motion to the alpha-domain, although the N-terminal residues are more flexible. Our results may provide useful information for understanding the structure-function relationship of human MT-3. |
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Keywords: | AD Alzheimer’s disease GST glutathione-S transferase HMQC heteronuclear multiple-quantum coherence HSQC heteronuclear single-quantum coherence MTs metallothioneins MT-3 metallothionein-3 NFT neurofibrillar tangles NOE nuclear Overhauser effect NOESY nuclear Overhauser enhancement spectroscopy RMSD root mean square deviation SP senile plaques TOCSY total correlation spectroscopy |
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