| 鹿茸血肽疏水性分离及抗氧 化和ACE抑制作用的研究 |
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| 引用本文： | 任建东,施用晖,乐国伟,马立芹,尹晓平,姜红. 鹿茸血肽疏水性分离及抗氧 化和ACE抑制作用的研究[J]. 天然产物研究与开发, 2010, 22(2) |
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| 作者姓名： | 任建东 施用晖 乐国伟 马立芹 尹晓平 姜红 |
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| 作者单位： | 1. 江南大学食品学院营养与食品安全研究所,无锡,214122
2. 江南大学食品科学与技术国家重点实验室;江南大学食品学院营养与食品安全研究所,无锡,214122 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 实验采用DA201-C型大孔吸附树脂对Alcalase蛋白酶水解鹿茸血3 h的水解液进行吸附,采用25%、50%、75%、100%乙醇分级洗脱,收集各组分进行氨基酸组成分析,发现各洗脱组分具有不同疏水性值,同时测定各组分的血管紧张素转化酶(ACE)抑制活性和二苯代苦味肼基自由基(DPPH·)清除活性:75%乙醇洗脱组分的ACE抑制活性最高,为54.71%,且ACE抑制活性与组分疏水性值显著相关;100%乙醇洗脱组分的DPPH清除率最高.
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| 关 键 词： | 鹿茸血 大孔吸附树脂 疏水性 ACE抑制活性 DPPH自由基 DPPH· |
Study on the Hydrophobic Separation,ACE Inhibitory Activity and Antioxidative Activity of Red Deer Antler Blood Hydrolysate Peptides |
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| REN Jian-dong,SHI Yong-hui,LE Guo-wei,MA Li-qin,YIN Xiao-ping,JIANG Hong. Study on the Hydrophobic Separation,ACE Inhibitory Activity and Antioxidative Activity of Red Deer Antler Blood Hydrolysate Peptides[J]. Natural Product Research and Development, 2010, 22(2) |
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| Authors: | REN Jian-dong SHI Yong-hui LE Guo-wei MA Li-qin YIN Xiao-ping JIANG Hong |
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| Affiliation: | REN Jian-dong2,SHI Yong-hui1,2,LE Guo-wei1,MA Li-qin2,YIN Xiao-ping2,JIANG Hong21State Key Laboratory of Food Science , Technology,Jiangnan University,2Institute of Nutrition , Food Safety,School of Food Science , Technology,Wuxi 214122,China |
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| Abstract: | Red deer antler blood hydrolysates were prepared by enzymatic hydrolysis with Alcalase for 3 h.Macroporous adsorption resin were used for the fractionation of hydrophobic peptides from the hydrolysates by gradient ethanol elution.Amino acid analysis results suggested that different ethanol elution fractions have different hydrophobicities.75% ethanol fraction showed the highest inhibitory activity of angiotensin I-converting enzyme (ACE),and the ACE inhibitory activity has a significant correlation with hyd... |
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| Keywords: | red deer antler blood macroporous adsorption resin hydrophobic ACE inhibitory activity |
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