Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae |
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Authors: | Rossell Sergio van der Weijden Coen C Kruckeberg Arthur L Bakker Barbara M Westerhoff Hans V |
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Institution: | Department of Molecular Cell Physiology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, The Netherlands. |
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Abstract: | A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated both metabolically and hierarchically. The method is expounded in terms of its implications for diverse types of regulation. It is also fine-tuned for cases where isoenzymes catalyze the flux through a single metabolic step. |
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Keywords: | Functional genomics Regulation Starvation Glucose transport |
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