A Method for Histological Preparation of Undecalcified Bone Sections Containing Acrylic Bone Cement |
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| Authors: | Lars Nimb Jensen J Rgen Steen Jensen Klaus Gotfredsen |
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| Institution: |
a Department of Orthopedic Surgery, Rigshospitalet, Denmark
b Department of Prosthetic Dentistry, Royal Dental College, University of Copenhagen, Denmark |
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| Abstract: | An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and sections of 50-100 μm thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylin-eosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification. |
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| Keywords: | bone cement biocompatibility and polymethylmethacrylate (PMMA) histological evaluation |
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