Selection of antibiotic-resistant mutants with enhanced isoamylase activity in Pseudomonas amyloderamosa |
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Authors: | Dong-Her Wu Chiou-Yen Wen Wen-Shen Chu Long-Liu Lin Wen-Hwei Hsu |
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Affiliation: | (1) Food Industry Research and Development Institute, P. O. Box 246, Hsinchu, Taiwan, R. O. C. |
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Abstract: | Summary Isoamylase-hyperproducing strains of Pseudomonas amyloderamosa were bred by mutagenesis with UV light and N-methyl-N-nitro-N-nitrosoguanidine (NTG). The selection criterion for such strains was based on the formation of large turbid zones around the bacterial colonies in agar medium containing antibiotics and 1% waxy corn starch. Mutant WN6410 was obtained by treating P. amyloderamosa JD210 with five cycles of 1 × 104 J UV light and one cycle of NTG. P. amyloderamosa WN6410 had 22-fold increase in isoamylase activity when compared to wild-type strain SB15 and the maximal enzyme activity, 5,100 U/ml, could be achieved within 48 h in 2.5 L fed-batch fermentation. |
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