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Restriction endonucleases in Azospirillum |
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| Authors: | G Schwabe G Posseckert W Klingmüller |
| Institution: | Department for Genetics, University of Bayreuth P.O. Box 3008, D-8580 Bayreuth F.R.G. Tel. (21) 552709 |
| Abstract: | Azospirillum brasilense, A. amazonense, and A. lipoferum strains were screened for restriction endonucleases using phage lambda DNA. The extract of A. brasilense 29711 cleaved lambda DNA into specific fragments. It was concluded that this strain possesses a class II restriction endonuclease which was named AbrI. AbrI has a single recognition site on lambda DNA at position of approx. 33 500 bp. AbrI was characterized as an isoschizomer of XhoI, which cuts lambda DNA at 33 498 bp and cleaves double-stranded DNA at the sequence 5'-C TCGAG-3'. From other Azospirilla strains only A. amazonense QRZ42 extracts (AamI activity) cleaved DNA into specific fragments under certain conditions. |
| Keywords: | Recombinant DNA bacteriophage λ Ad2 adenovirus 2 Ap ampicillin bp base pair(s) βME β-mercaptoethanol Cb carbenicillin EtBr ethidium bromide kb kilobases or 1000 bp Km kanamycin LB Luria broth PAGE polyacrylamide gel electrophoresis resistance u units |
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