Evaluation of Elisa test for therapeutic monitoring of Nelfinavir in HIV-positive patients |
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| Authors: | Uglietti Alessia Genco Francesca Donadel Elena Rinaldi Serena Bastiani Erica Maserati Renato Meroni Valeria |
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| Affiliation: | Infectious Disease Department University of Pavia, Foundation IRCCS San Matteo Hospital, Pavia, Italy. |
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| Abstract: | Therapeutic drug monitoring (TDM) is an important tool in the management of antiretroviral (ARV) therapy. The gold standard for measuring drugs plasma levels is High-Performance Liquid Chromatographic Assay (HPLC) however it is technically-demanding and time-consuming. We evaluated a new immunoenzymatic test (TDM-ELISA, Biostrands, Trieste, Italy) for nelfinavir and its active metabolite M8 in comparison with HPLC. A statistically significant difference in Ctrough between the two different tests was demonstrated but this difference was no longer significant when a value of 29% due to M8 aliquot was deleted. This faster TDM-ELISA may have an important role for TDM in HIV patients taking ARVs. |
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