蛋白质检测新技术——邻位连接技术及初步应用

邻位连接技术（proximity ligation assay，PLA），是新研发的一项高灵敏度的蛋白质体外分析技术。该方法利用一对邻位探针（proximity probes）对靶分子进行双识别，通过连接反应产生可扩增的检测信号，以实时 PCR进行放大和检测，将对蛋白质的检测转变成为对DNA的检测，实现痕量蛋白的分析，具有极高的检测灵敏度和特异性。综述了邻位连接技术的原理、研究进展以及该技术在蛋白质分析及疾病诊断领域的初步应用。

The New Technique for Protein Analyses: Proximity Ligation Assay and Its Application

Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which a pair of proximity probes are designed to bind pairwise to target proteins via a DNA ligation reaction of oligonucleotides results in the formation of an amplifiable DNA strand suitable for analysis. It allows extremely sensitive and specific detection of  proteins by allowing the protein targets to be represented as DNA signatures that may be subsequently amplified and detected by methods like PCR. Here the basic principle and the potential applications of PLA were discussed, as well as the preparation of proximity probes.