Ca2+ release by inositol trisphosphate from Ca2+-transporting microsomes derived from uterine sarcoplasmic reticulum |
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| Authors: | M E Carsten J D Miller |
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| Affiliation: | 1. Center for Nutrition & Health, Universidad Europea del Atlantico (UEA), Santander, Spain;2. Area de Salud y Nutriciòn, Universidad Internacional Iberoamericana (UNINI), Campeche, Mexico;3. Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A) Università Politecnica delle Marche, Ancona, Italy;4. Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez. Biochimica, Università Politecnica delle Marche, Ancona, Italy |
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| Abstract: | Microsomes derived from pregnant uterine sarcoplasmic reticulum, isolated by differential and sucrose density gradient centrifugation, accumulates Ca2+ in the presence of ATP. Inositol trisphosphate caused release of this Ca2+, in a dose dependent manner. 40% of the Ca2+ that can be released by the ionophore A23187 was released by 5 microM inositol trisphosphate. Removal of Mg by EDTA prior to addition of inositol trisphosphate did not change the course of Ca2+ release. These results indicate that by mobilizing intracellular Ca2+, inositol trisphosphate may be the link between hormonal stimuli and smooth muscle contraction. |
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