Particulate protein-synthesis factors associated with translatable mRNA in mouse hybridoma cells |
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| Authors: | Petr Pohlreich Frantisˇek Franeˇk Lubor Sˇimek Jan Hradec |
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| Institution: | 1. Second Department of Medical Chemistry and Biochemistry, Charles University, 128 53 Prague 2, Czechoslovakia;2. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 142 20 Prague 4, Czechoslovakia;3. Department of Biochemistry, Oncological Institute, 180 00 Prague 8, Czechoslovakia |
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| Abstract: | Besides of mRNA, the postribosomal pellet of mouse hybridoma cells contains RNA species which become labeled more rapidly than rRNA. Their synthesis is inhibited by actinomycin D. Density-gradient centrifugation of the postribosomal pellet yielded fractions of approx. 55–60 and 90S, synthesizing after the addition of both ribosomal subunits and energy-sources light and heavy chains of immunoglobulin, as demonstrated by indirect immunoprecipitation. Analysis of translation products by electrophoresis indicated the presence of precursors of mRNAs for immunoglobulin chains in these particles. Postribosomal pellets thus apparently contain different particles composed of similar polypeptide chains and containing protein-synthesis factors associated with translatable mRNA. |
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| Keywords: | mRNP messenger ribonucleoprotein |
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