A new purification procedure for <span class="math"><math><mtext>Clostridium difficile</mtext></math></span> enterotoxin期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A new purification procedure for $Clostridium difficile$ enterotoxin

Authors:	B. Rihn J.M. Scheftel R. Girardot H. Monteil

Affiliation:	Institut de Bactériologie de la Faculté de Médecine - Université Louis Pasteur 3, rue Koeberlé - 67000 Strasbourg - France

Abstract:	$Clostridium difficile$ produces two toxins, an enterotoxin and a cytotoxin. The enterotoxin was purified using fast methods (tangential flow filtration, fast protein liquid chromatography). The purified enterotoxin is composed of two subunits (A₁ = 41,500, A₂ = 16,000) and its pI is 3.5.

Keywords:	APC antibiotic-associated pseudomembranous colitis C Clostridium FPLC fast protein liquid chromatography IEF isoelectric focusing MW molecular weight PAGE polyacrylamid gel electrophoresis
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