Phosphatidylinositol 4,5-bisphosphate: Light-mediated breakdown in the vertebrate retina |
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Authors: | Abdallah Ghalayini Robert E. Anderson |
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Affiliation: | Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030 USA |
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Abstract: | Isolated frog () retinas were labeled in the dark with either [32P]PO4-orthophosphate or -[2-3H]inositol for 2.5–4 hrs. After washing the retinas with cold buffer, they were exposed to brief flashes of light (5 secs or 15 secs) and their rod outer segments isolated. Upon separation of labeled phospholipids, a specific decrease in label in phosphatidylinositol 4,5-bisphosphate was observed, whereas there was no significant effect on the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol, or phosphatidic acid. These results are indicative of a light-activated phosphatidylinositol 4,5-bisphosphate-specific phospholipase C in frog rod outer segments. |
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Keywords: | phosphatidylinositol 4,5-bisphosphate PIP phosphatidylinositol 4-phosphate PI phosphatidylinositol PA phosphatidic acid inositol 1,4,5-trisphosphate DG 1,2-diacylglycerol ROS rod outer segment RBP Ringer's bicarbonate pyruvate |
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