Melittin-stimulated arachidonic acid metabolism by cultured malignant human epidermal keratinocytes |
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Authors: | Robert H. Rice Lawrence Levine |
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Affiliation: | 1. Charles A. Dana Laboratory of Toxicology, Harvard School of Public Health Boston, Massachusetts 02115 USA;2. Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254 USA |
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Abstract: | Upon melittin stimulation, cultured SCC-13 keratinocytes release prostaglandins E2, F2α, 6-keto-F1α, thromboxane B2, leukotriene B4, and 6-sulfido-peptide-containing leukotrienes (SRS) into serum free medium. Release of prostaglandins E2, F2α, and SRS, normalized to cell protein, is 3- to 10-fold higher from rapidly growing than confluent cultures. Cells growing with hydrocortisone in the medium produce approximately twice the level of the cyclooxygenase-mediated metabolites PGE2 and PGF2α as those without hydrocortisone, but similar levels of the lipoxygenase-mediated metabolite SRS. The results demonstrate the potential utility of squamous carcinoma lines for investigating biochemical pathways of arachidonic acid metabolism in keratinocytes. |
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Keywords: | SRS 6-sulfidopeptide-containing leukotrienes |
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