Trihydroxytetraenes: A novel series of compounds formed from arachidonic acid in human leukocytes |
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Authors: | Charles N. Serhan Mats Hamberg Bengt Samuelsson |
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Affiliation: | Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden |
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Abstract: | Addition of 15L-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) to human leukocytes led to the formation of a novel series of compounds containing four conjugated double bonds. The yield of tetraenes was increased approx. 100-fold when ionophore A23187 (5 μM) was added simultaneously with 15-HPETE. The structure of the major tetraene was established by physical methods as well as by chemical degradation and found to be 5,6,15L-trihydroxy-7,9,11,13-eicosatetraenoic acid. |
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Keywords: | 15-HPETE 15L-hydroperoxy-5,8,11,13-eicosatetraenoic acid 5,15-DHETE 5,15-dihydroxy-6,8,11,13-eicosatetraenoic acid 8,15-DHETE 8,15-dihydroxy-5,9,11,13-eicosatetraenoic acid 14,15-DHETE 14,15-dihydroxy-5,8,10,12-eicosatetraenoic acid TLC thin layer chromatography RP-HPLC reversed phase high performance liquid chromatography trimethylsilyl |
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