Winter wheat mitochondria functioning in vitro in the presence of calcium ions and stress uncoupling CSP310 protein |
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Authors: | A V Kolesnichenko O I Grabel’nykh T P Pobezhimova V V Turchaninova V K Voinikov |
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Institution: | (1) Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia |
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Abstract: | The effects of different Ca2+ concentrations on winter wheat (Triticum aestivum L.) functioning and cytochrome c release after organelle incubation with cold-shock protein with a mol. wt of 310 kD or after cold shock were studied. Low (1–5 μM) and high (25–50 μM) Ca2+ concentrations inhibited mitochondrial respiration in control seedlings, whereas 10 μM Ca2+ enhanced respiration in state 4 and reduced indices characterizing coupling (respiratory control (RC) and ADP: O ratio). At concentrations of 6–20 and 50 μM, Ca2+ ions suppressed CSP310 uncoupling effect, which reduced the rate of respiration and an increase in the RC and ADP: O ratio. Low-temperature stress and exogenous CSP310 induced cytochrome c leakage from winter wheat mitochondria both in the absence of Ca2+ and in the presence of its low concentrations. |
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Keywords: | Triticum aestivum mitochondria uncoupling of oxidation and phosphorylation cold shock protein with a mol wt of 310 kD respiratory control Ca2+ cytochrome c |
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