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A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs |
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| Authors: | Karamjit Singh Dolt Melanie L. Lawrence Eve Miller-Hodges Joan Slight Anna Thornburn Paul S. Devenney Peter Hohenstein |
| Affiliation: | 1. MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.; 2. The Roslin Institute, University of Edinburgh, Midlothian, United Kingdom.; Johns Hopkins School of Medicine, United States of America, |
| Abstract: | There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models. |
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