Microwave-mediated enzyme-linked immunosorbent assay procedure |
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Authors: | Nahar Pradip Bora Utpal Sharma Gainda L Kannoujia Dileep Kumar |
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Institution: | Institute of Genomics and Integrative Biology (CSIR), Delhi 110007, India. pnahar@igib.res.in |
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Abstract: | Here we demonstrate a novel microwave-mediated enzyme-linked immunosorbent assay (MELISA) method that has dramatically reduced the enzyme-linked immunosorbent assay (ELISA) timing to less than 5 min with a result comparable to that obtained by 18-h conventional ELISA. Efficacy of the MELISA procedure is demonstrated by detecting human immunoglobulin G (IgG), rabbit IgG, human immunoglobulin E (IgE), human interleuken 1β (IL-1β), Entamoeba histolytica antibody, and Aspergillus fumigatus antibody. MELISA could be an excellent substitute for time-consuming conventional ELISA for rapid diagnosis of diseases in cases of medical urgency, outbreak of infectious diseases, and screening of samples in blood banks or emigration counters. |
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