Institution: | 1 School of Wool & Pastoral Sciences, University of N.S.W., Kensington, N.S.W., 2033, Australia 2 School of Biochemistry, University of N.S.W., Kensington, N.S.W., 2033, Australia 3 Division of Animal Health, C.S.I.R.O., McMaster Laboratory, Private Bag No. 1, P.O., Glebe, N.S.W., 2037, Australia |
Abstract: | The dissimilation of leucine, isoleucine and valine to volatile fatty acids was determined in Fasciola hepatica and the degradation of (U?14C) branched amino acids to the volatile fatty acids end products demonstrated. F. hepatica was found to metabolize leucine, isoleucine and valine to isovaleric, 2-methylbutyric and isobutyric acid respectively. The rate of formation of isobutyrate, isovalerate and 2-methylbutyrate was found to be positively related to the rate of propionic acid production with air or nitrogen as the gas phase. However, under 95% O2/5% CO2 the formation of the branched chain acids was independent of propionic acid production. The production of isobutyrate, isovalerate and 2-methylbutyrate caused a simultaneous reduction in the rate of acetate formation. The role of propionate formation in regulating metabolism is discussed. |