Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli |
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| Authors: | Crawford, IP Wilde, A Yelverton, EM Figurski, D Hedges, RW |
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| Affiliation: | Department of Microbiology, University of Iowa, Iowa City 52242. |
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| Abstract: | The genes for the large and small subunits of anthranilate synthase (trpEand trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174into E. coli by R-prime formation with the broad-host- range plasmidR68.44. Sequential subcloning into plasmid vectors reduced the activePseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence thatthis region contains the promoter for its own expression and retains avestigial regulatory response to tryptophan scarcity or excess. |
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