| 禾谷类作物雄性不育及育性恢复表达载体的构建 |
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| 引用本文： | 张晓国,刘玉乐,杨清平,田波.禾谷类作物雄性不育及育性恢复表达载体的构建[J].武汉植物学研究,2000,18(1):15-20. |
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| 作者姓名： | 张晓国 刘玉乐 杨清平 田波 |
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| 作者单位： | 1. 武汉大学生命科学学院,武汉,430072
2. 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 国家“8 63”计划，武汉市晨光计划资助的课题 |
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| 摘 要： | 通过ＰＣＲ反应扩增出了玉米花药特异启动子ＣＡ５５，将其分别与Ｂａｒｎａｓｅ和Ｂａｒｓｔａｒ基因融合，构建成了植物雄性不育基因ＣＡ５５ＢＮＮＯＳ和其育性恢复基因ＣＡ５５ＢＳＮＯＳ，再将它们分别插入到ｐＣＡＭＢＩＡ３３００中，获得了应用于禾谷类作物的基因工程雄性不育及育性恢复的表达载体。
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| 关 键 词： | 雄性不育 育性恢复 表达载体 禾谷类作物 |
| 收稿时间： | 1998-10-18 |
| 修稿时间： | 1998-12-30 |
CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION |
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| Zhang Xiaoguo,Liu Yule,Yang Qingping,Tian Bo.CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION[J].Journal of Wuhan Botanical Research,2000,18(1):15-20. |
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| Authors: | Zhang Xiaoguo Liu Yule Yang Qingping Tian Bo |
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| Institution: | 1. College of Life Sciences, Wuhan University Wuhan 430072;2. Institute of Microbiology, The Chinese Academy of Sciences Beijing 100080 |
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| Abstract: | The tapetum- promoter( CA55) was amplified from the total DNA of Zea mays L.by polymerase chain reaction,and was fused with barnase( BN) and barstar( BS) genes,respectively.The male sterile gene.CA55BNNOS and its fertility restoration gene CA55BSNOS were construced.They were cloned into p CAMBIA330 0 ,respectively.The expression vectors for cereal male sterility and its fertility restoration were obtained. |
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| Keywords: | Male sterility Fertility restoration Expression vector Cereal |
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