药用植物刺山柑愈伤组织诱导及细胞生长代谢特征研究

本文研究了不同外植体及激素对刺山柑愈伤组织诱导的影响,不同激素配比对愈伤组织增殖培养以及悬浮细胞的生长与代谢特征.结果表明:以刺山柑叶片作为诱导愈伤组织的材料,效果较佳;愈伤组织诱导和继代的适宜培养条件是分别是MS+0.5 mg/L 2,4-D+1.0mg/L6-BA和MS+1.0mg/L2,4-D+1.5mg/L6-BA.刺山柑悬浮培养细胞的生长周期为30天左右,细胞生长曲线呈"S"形,生物量增长2.8倍左右;细胞生长周期内,碳源消耗规律表现为蔗糖和可溶性总糖的浓度持续降低,而还原糖则表现为先升高后降低;过氧化物酶活测定显示酶活水平与蔗糖浓度的高低呈一定程度的正相关.

Research on the Callus Induction and the Cell Growth and Metabolism Characteristics of Capparis spinosa L.

Different kinds of explants and plant hormones were applied to study their effects on the callus induction of Capparis spinosa L., and different proportions of plant hormones were also employed to explore the effects on callus propagation as well as the growth and metabolism characteristics of suspension cells. These results revealed that, the fresh leaf was the best explant for inducing the callus formation, and MS medium with 0.5 mg/L 2, 4-D+1.0 mg/L 6-BA and 1.0mg/L 2, 4-D +1.5 mg/L6-BA was optimum for callus in-duction and subculture. The growth cycle was about 30 days and the growth showed curve of"S" type, the proliferation rate was about 2.8 times. On the other hand, during the growth cycle of the suspension cell, concentrations of both sucrose and total soluble sugar were decreased steadily, concentration of reducing sugars was first increased and then decreased, and POD activity was accordingly posi-tive-correlated with the concentration of sucrose.