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Induced accumulation of oleanolic acid and ursolic acid in cell suspension cultures of <Emphasis Type="Italic">Uncaria tomentosa</Emphasis> |
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| Authors: | Iris?Feria-Romero Elizabeth?Lazo Teresa?Ponce-Noyola Carlos?M?Cerda-García-Rojas |
| Institution: | (1) Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Av. Instituto Politécnico Nacional, 2508, C. P. 07360, México, D. F., Mexico;(2) Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Av. Instituto Politécnico Nacional, 2508, C. P. 07360, México, D. F., Mexico |
| Abstract: | Increasing sucrose from 20 to 50 g l−1 in Uncaria tomentosa cell suspension cultures enhanced ursolic acid and oleanolic acid production from 129 ± 61 to 553 ± 193 μg g−1 cell dry wt. The maximal concentration of both triterpenes (1680 ± 39 μg g−1 cell dry wt) was 8 days after elicitation by jasmonic acid, while yeast extract or citrus pectin treatments produced 1189 ± 20 or 1120 ± 26 μg g−1 cell dry wt, respectively. The ratio of ursolic acid:oleanolic acid was constant at 70:30. |
| Keywords: | cell cultures jasmonic acid sucrose supply Uncaria tomentosa ursolic and oleanolic acids |
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