Genetic polymorphisms of GSTO2, GSTM1, and GSTT1 and risk of gastric cancer |
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Authors: | Masoudi Mohammad Saadat Iraj Omidvari Shahpour Saadat Mostafa |
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Institution: | (1) Department of Biology, College of Sciences, Shiraz University, Shiraz, 71454, Iran;(2) Institute of Biotechnology, Shiraz University, Shiraz, Iran;(3) Department of Chemotherapy, Shiraz University of Medical Sciences, Shiraz, Iran |
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Abstract: | Objective The glutathione S-transferases (GSTs) are a superfamily of proteins that participates in detoxification. The GSTs were dividing into several
classes including omega (GSTO), mu (GSTM) and theta (GSTT) classes. In human GSTO2, GSTM1, and GSTT1 are polymorphic. In order to study whether GSTO2, GSTM1, and GSTT1 polymorphisms are associated with increased gastric cancer risk in Iranian patients, the present case–control study was done.
Methods Genomic DNA was extracted from peripheral blood of 67 gastric cancer patients and 134 control subjects. The genotyping was
performed using PCR-based method. The possible association of gastric cancer with the GSTO2 N142D polymorphism was estimated with assuming additive, dominant, and recessive effect of the variant 142D allele. To investigate whether profiles of GST genotypes are associated with the risk of gastric cancer, we used unconditional
logistic regression analysis. Results The GSTO2 142D allele in additive, dominant and recessive models was not associated with the risk. Because GSTM1, GSTT1, and GSTO2 genes belong to low-penetrance genes which might be involved in the carcinogenesis, patients and controls without family
of cancer in first-degree relatives were also analyzes separately. To investigate whether profiles of GST genotypes are associated
with the risk of gastric cancer, we used unconditional logistic regression analysis with GSTM1, GSTT1, and GSTO2 genotypes as predictor factors. The GSTO2 DD genotype was associated with decreased risk as compared to GSTO2 NN genotype (OR = 0.21, 95% CI: 0.05–0.92, P = 0.038). Conclusions Present findings show that GSTO2 DD genotype decreases the risk of gastric cancer in individuals without history of cancer in their first-degree relatives. |
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Keywords: | :" target="_blank">: Gastric cancer Genetic polymorphism GSTM1 GSTO2 GSTT1 Iran |
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