Heptapeptide toxin production during the batch culture of two Microcystis species (Cyanobacteria) |
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Authors: | Mariyo F Watanabe Ken-Ichi Harada Kennji Matsuura Masayuki Watanabe Makoto Suzuki |
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Institution: | 1. Tokyo Metropolitan Research Laboratory of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo, 169, Japan 2. Faculty of Pharmacy, Meijo University, Tempaku, Nagoya, 468, Japan 3. National Science Museum, 4-1-1, Amakubo, Tsukuba, Ibaraki, 305, Japan
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Abstract: | Changes in the content of cyclic heptapeptide hepatotoxins called microcystins were investigated during batch culture of two
Microcystis species using high performance liquid chromatography. After adsorption to ODS-silica gel cartridges and elution with methanol,
the toxins were analyzed and quantified by HPLC. 35 μg per 100 mg dry cells of microcystin-RR, 34 μg of -YR and 43 μg of -LR
were present at the beginning of the exponential growth phase of M. viridis. Microcystin-RR increased markedly towards the end of the exponential phase with the maximum content of 112 μg per 100 mg
cells was measured at the late stage of the exponential phase. A remarkable increase of microcystin-YR from 130 μg per 100
mg cells to 1020 μg was observed during the exponential phase of a highly toxic strain of M. aeruginosa. However no clear differences were found in the pattern of change among the three toxins during the growth course. |
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Keywords: | toxin Cyanobacteria Microcystis culture |
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