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Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving |
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| Authors: | Panagiotou Vasiliki Love Kerry Routenberg Jiang Bo Nett Juergen Stadheim Terrance Love J Christopher |
| Institution: | 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139;2High Throughput Screening and Strain Selection Group, GlycoFi, Inc., Lebanon, New Hampshire 03766;3Strain Development Group, GlycoFi, Inc., Lebanon, New Hampshire 03766;4The Eli and Edythe L. Broad Institute, Cambridge, Massachusetts 02142 |
| Abstract: | The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain. |
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