| 分离纯化后的烟草叶片液泡的显微观察 |
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| 引用本文： | 鲁云霞,王延枝.分离纯化后的烟草叶片液泡的显微观察[J].植物研究,2003,23(1):36-38,T002. |
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| 作者姓名： | 鲁云霞 王延枝 |
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| 作者单位： | 1. 安徽医科大学生化教研室,合肥,230032
2. 武汉大学生命科学学院,武汉,430072 |
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| 摘 要： | 将分离纯化后的烟草叶片注佻 分别置于光镜和电镜下观察得到原生质体，原生质体释放液泡的过程，纯化后液泡的相应照片，结果说明我们选用的方法能得到完整的原生质体和液泡，且中央液泡是成熟植物细胞中体积最大的细胞器。
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| 关 键 词： | 分离纯化 烟草 叶片 液泡 显微观察 原生质体 光镜 电镜照片 |
MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES |
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| LU Yun-Xia,WANG Yan-Zhi.MICROSCOPIC OBSERVATION OF VACUOLES ISOLATED AND PURIFIED FROM TOBACCO LEAVES[J].Bulletin of Botanical Research,2003,23(1):36-38,T002. |
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| Authors: | LU Yun-Xia WANG Yan-Zhi |
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| Institution: | LU Yun-Xia1 WANG Yan-Zhi2 |
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| Abstract: | Observing vacuoles isolated and purified from tabacco leaves under optic and electronic microscopes, we obtained photographs of protoplasts, process of vacuoles released from protoplasts and vacuoles after purification. The results show that the method we used could obtain complete protoplasts and vacuoles,and the central vacuole is the largest organelle in one mature plant cell. |
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| Keywords: | tobacco protoplasts vacuoles photographs under optic and electronic microscopes |
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