Adrenal tissue in the male African catfish,Clarias gariepinus: localization and steroid hormone secretion |
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Authors: | Vermeulen G. J. Lambert J. G. D. Teitsma C. A. Zandbergen M. A. Goos H. J. T. |
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Affiliation: | (1) Department of Experimental Zoology, Research Group for Comparative Endocrinology, University of Utrecht, Padualaan 8, NL-3584 CH Utrecht, The Netherlands |
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Abstract: | Interrenal-like tissue in male African catfish is localized in paired organs, situated retroperitoneally anterior of the kidney. Histological and enzyme-histochemical (3-hydroxysteroid dehydrogenase) reactions were carried out on sections of these organs in order to localize steroid-producing cells. In vitro incubations were carried out to determine the steroidogenic capacity of the interrenal-like tissue. Twenty-one steroids could be identified and quantified in the incubation medium, by means of gas chromatography followed by mass spectrometry. Cortisol, 5-and 5-androstanedione and androstenedione together comprised about 80% of the total steroid content. In a previous study, twenty testicular steroids were detected in plasma after castration. In the present investigation we have shown that most of these steroids can be produced by the interrenal-like tissue, which thus can be considered as an extra-testicular source of gonadal steroids. |
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Keywords: | Adrenals Morphology Steroids Gas chromatography Mass spectrometry Clarias gariepinus (Teleostei) |
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